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KMID : 0525720160210040236
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Journal of Chitin and Chitosan
2016 Volume.21 No. 4 p.236 ~ p.241
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Anti-inflammatory Effect of an Ecklonia cava Ethanol Extract in Macrophage via Inhibition of the NF-κB/MAPK Signal Pathways
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Kim Mi-Kyoung
Kim Dae-Yong
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Abstract
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Ecklonia cava is a brown alga that evidences antioxidant, diarrhea and anticoagulant effect. However, the molecular mechanisms underlying its anti-inflammatory effects remain poorly understood. In the current study, the effect of Ecklonia cava ethanol extracts (ECEE) on lipopolysaccharide (LPS)-induced cytokine expression in RAW264.7 cells was investigated. Our results indicate that ECEE inhibits LPS-induced inducible nitric oxide (iNOS) and cyclooxygenase (COX)-2 in a concentration-dependent manner. Also, pre-treatment with ECEE significantly inhibited the expression of inflammatory cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). Moreover, phosphorylation of mitogen-activated protein kinases (MAPKs) and nuclear translocation of nuclear factor-kappa B (NF-κB) were strongly suppressed by ECEE in LPS-stimulated RAW264.7 cells. These results indicate that ECEE has an anti-inflammatory effect in RAW264.7 macrophage through inhibition of the NF-κB/MAP kinase signal pathways.
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KEYWORD
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Ecklonia cava, Lipopolysaccharide, Inducible nitric oxide, Cyclooxygenase-2, Nuclear factor-κB
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